The right three images were prepared using QuPath v0.6.0.

1. Background

Name：Kang Wu

E-mail：wu__kang@126.com

ORCID：0000-0002-9024-795X

2. Education

1. 2007-09 ~ 2012-07，Biochemistry and Molecular Biology (Ph.D), Shanghai Jiao Tong University
1. 2001-09 ~ 2007-07，Biotechnology (joint program of bachelor and master), Northwest A&F University

3. Experience

1. 2022-07 ~ 2026-03，R&S Xunyi，senior director of R&D
1. 2021-08 ~ 2022-06，Matridx，medical scientist
1. 2021-06 ~ 2021-08，PersonalBio，R&D project manager
1. 2012-07 ~ 2021-06，Shanghai Public Health Clinical Center，research assistant

4. 💩Journal articles

4.1 (co-)1st author SCI (# co-first author，*corresponding author)

1. Xu JC#, Wu K#, Ma RQ, Li JH, Tao J, Hu ZD, Fan XY*. Establishment of an in vitro model of monocyte-like THP-1 cells for trained immunity induced by bacillus Calmette-Guérin. BMC Microbiol. 2024;24:130.
1. Shi JC#, Wu WJ#, Wu K#, Ni CR, He GQ, Zheng SL, Cheng F, Yi YX, Ren RT*, Jiang XG*. The diagnosis of leptospirosis complicated by pulmonary tuberculosis complemented by metagenomic next-generation sequencing: a case report. Front Cell Infect Microbiol. 2022;12:922996. (Case report).
1. Liu C#, Wu K#, Sun TY, Chen B, Yi YX, Ren RT*, Xie LX*, Xiao K*. Effect of invasive mechanical ventilation on the diversity of the pulmonary microbiota. Crit Care. 2022;26:252. (Review).
1. Wu K, Yan B, Douglas BL, Li T*, Fan XY*. Tailored co-localization analysis of intracellular microbes and punctum-distributed phagosome-lysosome pathway proteins using ImageJ plugin EzColocalization. Sci Rep. 2021;11:1096.
1. Wu K, Li M, Chen ZY, Douglas BL*, Fan XY*. Probe signal values in mRNA arrays imply an excessive involvement of neutrophil FCGR1 in tuberculosis. Front Med. 2020;7:19.
1. Pei ZL#, Wu K#, Li ZH#, Li CQ#, Zeng L, Li F, Pei N, Liu HM, Zhang SL, Song YZ, Zhang XY, Xu JQ, Fan XY*, Wang J*. Pharmacologic ascorbate as a pro-drug for hydrogen peroxide release to kill mycobacteria. Biomed Pharmacother. 2019;109:2119-2127.
1. Wu K#, Wong KW#, Deng WL, Zhang H, Li J, Lowrie DB*, Fan XY*. The numerical predominance and large transcriptome differences of neutrophils in peripheral blood together inevitably account for a reported pulmonary tuberculosis signature. Int J Genomics. 2017;2017:5830971.
1. Wu K#, Zhao XJ#, Wong KW, Fan XY*. Comparison of plasmid DNA versus PCR amplified gene of insert DNA for nucleofection in Kasumi-1 cells. Cytotechnology. 2015;67(2):275-283.
1. Wu K, Fang H, Lyu LD, Lowrie DB, Wong KW*, Fan XY*. A derived network-based interferon-related signature of human macrophages responding to Mycobacterium tuberculosis. Biomed Res Int. 2014;2014:713071.
1. Ma H#, Wu K#, Liu F Yang H, Kang H, Chen NN, Yuan Q, Zhou WJ, Fan XY*. Dose of incorporated immunodominant antigen in recombinant BCG impacts modestly on Th1 immune response and protective efficiency against Mycobacterium tuberculosis in mice. J Immunol Res. 2014;2014:196124.
1. Jin W#, Wu K#, Li YZ# Yang WT, Zou B, Zhang F, Zhang J*, Wang KK*. AML1-ETO targets and suppresses cathepsin G, a serine protease, which is able to degrade AML1-ETO in t(8;21) acute myeloid leukemia. Oncogene. 2013;32(15):1978-1987.
1. Wu K#, Dong DD#, Fang H#, Levillain F#, Jin W, Mei J, Gicquel B, Du Y, Wang K, Gao Q, Neyrolles O*, Zhang J*. An interferon-related signature in the transcriptional core response of human macrophages to Mycobacterium tuberculosis infection. PLoS One. 2012;7(6):e38367.

5. 💩Patents

1. 张腾、吴康、纪逸群、东亚娟、钟军、罗卫峰、范小勇、赵建华。一种改变不同核酸靶标相对PCR扩增速度的方法。发明专利。申请号：202410372505.4。申请日期：2024.03.29。_该专利在2024.06.18被驳回。本人自认为该专利有有趣的发现，即通过具体实验证实了带有通用引物序列的靶标特异引物对的qPCR扩增性能更好（扩增体系亦包括通用引物对。相比于未带通用引物序列的靶标特异引物对的qPCR），进而能够较好实现改变不同核酸靶标的相对PCR扩增速度的目的。
1. 吴康、张腾、纪逸群、钟军、罗卫峰、范小勇、赵建华。一种通过预先PCR改变不同核酸靶标相对扩增速度的方法。发明专利。申请号：202410274764.3。申请日期：2024.03.11。
1. 吴康、赵建华、张腾、范小勇、罗卫峰、东亚娟、纪逸群。基于人基因组扩增子的illumina测序文库构建的引物组合。发明专利。授权公告号：CN117701691B。申请日期：2024.02.01。
1. 吴康、东亚娟、张腾、范小勇、罗卫峰、赵建华、纪逸群。基于左侧探针退火和右侧探针退火延伸的核酸扩增方法。发明专利。授权公告号：CN117343999B。申请日期：2023.12.05。
1. 范小勇、吴康、罗道良。基于常规引物的针对串联重复序列的恒温扩增体系、扩增方法及常规引物对。发明专利。授权公告号：CN111269969B；申请日期：2020.03.06。
1. 范小勇、吴康、罗道良。重组卡介苗菌株、其制备方法和用途。发明专利。授权公告号：CN111979162B；申请日期：2019.05.22。
1. 范小勇、吕亮东、赵国屏、马辉、吴康。mazG基因缺失的抗结核重组卡介苗。发明专利。授权公告号：CN106148258B；申请日期：2015.04.20。
1. 吴康、黄家颖、范小勇。基于蛋白-蛋白相互作用网络的基因集鉴定方法。发明专利。授权公告号：CN104182654B；申请日期：2014.07.30。
1. 吴康、范小勇、黄家颖、赵旭杰。PCR扩增基因核转悬浮动物细胞的方法。发明专利。授权公告号：CN103740760B；申请日期：2014.01.09。专利权终止日期：2024.01.09。

6. 💩Scientific projects

序号	项目类型	项目号	负责人	期限	经费额(￥万)
5	舟山市普陀区一般科技计划项目	2023YB003	吴康	2023/01-2025/06	12
4	上海市科委医学引导	18411970700	吴康	2018/09-2021/09	20
3	国家重点研发计划	2018YFD0500900	吴康(子课题3)	2018/06-2021/09	47/1263
2	国自然青年	81301407	吴康	2014/01-2016/12	23
1	上海市科委青年	12ZR1448200	吴康	2012/10-2015/09	10